Staff Scientist - iPSC genetic manipulation

Staff Scientist - iPSC genetic manipulationApplylocations: Hinxton, Cambridgeshiretime type: Full timeposted on: Posted 15 Days Agojob requisition id: JR103728Do you want to help us improve human health and understand life on Earth? Make your mark by shaping the future to enable or deliver life-changing science to solve some of humanity's greatest challenges. The Opportunity: The Cellular Genomics Programme is seeking a highly motivated staff scientist to lead wet lab work in the Lee-Six group. The group is focused on studying the earliest stages of childhood cancer by investigating the conditions under which cells with particular cancer-driving mutations gain a selective advantage over their healthy neighbours. The applicant will have the opportunity to be embedded in a large community of experimental and computational scientists with access to world-leading computational and experimental facilities at the institute. About the Team: Henry Lee-Six is a paediatric pathology registrar. His previous work has been focused on somatic mutations in normal tissues. He is starting a new group in the Cellular Genomics Programme with the aim of understanding the interplay of somatic mutations and development in childhood cancer. A major goal of the group is to investigate the relative contribution of mutation identity, cell state, and microenvironmental context in determining whether a mutant cell progresses to form a tumour. The group pursues this line of research by studying samples of children's tumours and normal tissues with cutting-edge spatial transcriptomic platforms coupled to different mutation-calling technologies such as laser capture microdissection and duplex sequencing. These data are used to build models of what determines a cell's selective advantage, which are in turn queried to suggest perturbations to a cell's state or microenvironment that might change the relative fitness of a mutant clone. These in silico predictions are then tested in vitro using iPSC and cell-line models. The group is a mixture of wet and dry lab researchers and clinicians and collaborates closely with other teams in the Cellular Genomics Programme and the wider Institute. About the Role: You will use CRISPR-Cas9 to generate genotypes of interest in human iPSC lines, differentiate them into cell states of interest, and characterise their behaviour using a combination of methods, including transcriptomic analysis and functional assays of proliferation and death. In due course you will design and execute more complex culture systems, including co-culture. You will work closely with computational members of the team, and will collaborate with other teams within the Institute and beyond. You will contribute to the day-to-day supervision of students. About you: You are experienced in genetic manipulation and cell culture, and are able to design experiments, work independently, and troubleshoot. You will ideally have experience working with CRISPR manipulation of iPSC lines. You understand key concepts in genomics and are able to work with computational members of the team to improve experimental design and analysis. You are able to contribute to the smooth running of the lab and supervise students. Knowledge, skills and experience required: Technical Skills: PhD in relevant wet-lab subject (e.g. Molecular Biology, Developmental Biology) Post-doctoral experience including proven track record (publications and meetings) in field of expertise Experience using CRISPR editing tools Comprehensive, up to date and in depth understanding of CRISPR-based genetic screening, stem cell culture and differentiation Practical experience in mammalian ES or iPS Competencies and Behaviours: Ability to test, implement and troubleshoot new approaches and techniques Excellent communication skills Proven ability to work effectively within a team Self-motivated, able to be independent and organised Good attention to detail and record keeping for sample and experimental tracking An ability to lead and inspire others to achieve scientific projects Demonstrates inclusivity and respect for all Other Information: For further role details, please see role profile. Salary per annum (dependent upon skills and experience): £45,788-£54,397 Application Process: Please apply with your CV and a Cover letter outlining how you meet the criteria set out above and in the role profile. Closing Date: 2nd July 2026