Sequencing Laboratory Scientist

Organisation position and purpose

The activities of the High-Throughput Sequencing (HTS) unit are central to delivering high-impact genomics research at the Pirbright Institute. We were first established in 2018 and play a major role in executing small and large genomics projects at the institute. We are located within state-of-the-art high containment facilities within the Plowright building and deliver bespoke and directed assistance to users. We work closely with many research groups at Pirbright, in addition to assisting with WOAH/FAO reference laboratory pathogen surveillance duties. The unit has access to Illumina and Nanopore based sequencing technology platforms, in addition to liquid handling robots and single cell equipment. The Research Technician will be a pivotal member of the team and will support the core facility in delivering high quality genomics datasets. The position will perform key duties including (but not exclusive to) library preparation protocols, molecular genomics assays and maintenance of the sequencing equipment and robotic liquid handlers.

Main duties and responsibility Responsible for sample preparation of High throughput sequencing libraries, which will include: (50%)

• Preparation of samples for high-throughput sequencing using core library preparation SOPs (including, but not exclusive to amplicon, RNA seq, single cell and shotgun sequencing protocols)
• Working with automated laboratory robotics systems for sequencing library preparation
• Working with Illumina and Oxford nanopore technology platforms.
• Involved in core facilities genomics data management
• Quality control of sample libraries prior to library preparation and sequencing

Responsible for working as part of an established team, which will include: (25%)

• Communication of progress and data to scientific groups at institute and other members of the HTS core facility
• Assist with lab management (ordering, looking after equipment, maintenance, sample inventories)
• Supporting and training of collaborating groups at the institute on High-throughput sequencing technologies

Contribute to overall research culture at Pirbright, which will include: (25%)

• Service on committees, steering groups and other meetings as appropriate
• Take responsibility with others for the daily housekeeping of the laboratories
• Take responsibility with others for ensuring that work in the laboratory is conducted safely and in accordance with local health and safety policies

This job description is only an outline of the tasks, responsibilities and outcomes required of the role. The jobholder will carry out any other duties as may be reasonably required by his/her line manager. The job description and personal specification may be reviewed on an ongoing basis in accordance with the changing needs of the Institute.

Specific requirements

• A degree or equivalent in a relevant branch of biosciences
• Relevant bench experience
• Understanding of High-Throughput Sequencing
• Must be able to give meticulous attention to detail including the management of samples and recording of data
• Ability to assess resource requirements and use resources effectively
• Evidence of excellent oral and written communication skills, including the ability to communicate effectively with funding bodies, peer review groups, government agencies
• Experience of working in a high containment laboratory environment
• Experience with high-throughput sequencing
• Evidence of networking skills with the ability to build effective collaborative relationships
• Computer literate with a good knowledge of different computer programs and experience in data presentation

Due to the nature of the work carried out by Pirbright, all successful applicants will be required to go through a comprehensive screening process including reference and qualification checks, financial and security clearances.

What we offer

• Generous Group Personal Pension Scheme and life cover (subject to age and earnings)
• 25 days annual leave per annum, pro rata, plus public and privilege days
• Buy and sell annual leave
• Option to carry over up to two weeks annual leave
• Pirbright gym with subsidised membership
• Pirbright social club
• Pirbright Lifestyle Hub (employee discount site)